Feasibility of Imaging EpCAM Expression in Ovarian Cancer Using Radiolabeled DARPin Ec1.

Epithelial cell adhesionmolecule (EpCAM) is overexpressed in 55%-75% of ovarian carcinomas (OC). EpCAM might be used as a target for a treatment of disseminated OC. Designed ankyrin repeats protein (DARPin) Ec1 is a small (18 kDa) protein, which binds to EpCAM with subnanomolar affinity. We tested a hypothesis that Ec1 labeled with a non-residualizing label might serve as a companion imaging diagnostic for stratification of patients for EpCAM-targeting therapy.

Ec1 was labeled with 125I using N-succinimidyl-para-iodobenzoate. Binding affinity, specificity, and cellular processing of [125I]I-PIB-Ec1 were evaluated using SKOV-3 and OVCAR-3 ovarian carcinoma cell lines. Biodistribution and tumor-targeting properties of [125I]I-PIB-Ec1 were studied in Balb/c nu/nu mice bearing SKOV-3 and OVCAR-3 xenografts. EpCAM-negative Ramos lymphoma xenografts served as specificity control. Binding of [125I]I-PIB-Ec1 to ovarian carcinoma cell lines was highly specific and had affinity in picomolar range.

Slow internalization of [125I]I-PIB-Ec1 by OC cells confirmed utility of non-residualizing label for in vivo imaging. [125I]I-PIB-Ec1 provided 6 h after injection tumor-to-blood ratios of 30 ± 11 and 48 ± 12 for OVCAR-3 and SKOV-3 xenografts, respectively, and high contrast to other organs. Tumor targeting was highly specific.

Mouse SKT shRNA Plasmid

20-abx980555
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse Sickle tail protein (SKT) ELISA Kit

abx389166-96tests 96 tests
EUR 911

Mouse Sickle tail protein, Skt ELISA KIT

ELI-29595m 96 Tests
EUR 865

CD14 Recombinant Protein

96-108 0.1 mg
EUR 516.5
Description: Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.

RG 108

A1913-10 10 mg
EUR 119
Description: RG108 is a DNA methyltransferase (DMNT) inhibitor that enhanced reprogramming of OK-transduced MEFs in the presence of BIX. It is a small molecule that effectively blocked DNA methyltransferases in vitro and did not cause covalent enzyme trapping in human cell lines.

RG 108

A1913-25 25 mg
EUR 166
Description: RG108 is a DNA methyltransferase (DMNT) inhibitor that enhanced reprogramming of OK-transduced MEFs in the presence of BIX. It is a small molecule that effectively blocked DNA methyltransferases in vitro and did not cause covalent enzyme trapping in human cell lines.

RG 108

A1913-5.1 10 mM (in 1mL DMSO)
EUR 119
Description: RG108 is a DNA methyltransferase (DMNT) inhibitor that enhanced reprogramming of OK-transduced MEFs in the presence of BIX. It is a small molecule that effectively blocked DNA methyltransferases in vitro and did not cause covalent enzyme trapping in human cell lines.

RG 108

1679-10
EUR 180

RG 108

1679-30
EUR 359

Glucose M9Y

108 500 g
EUR 84

Human Sickle tail protein homolog, SKT ELISA KIT

ELI-18163h 96 Tests
EUR 824

Angiogenin (108-122)

HY-P1516 10mg
EUR 980

Angiogenin (108-122)

5-00673 4 x 1mg Ask for price

Angiogenin (108-123)

5-00674 4 x 1mg Ask for price

Angiogenin (108-123)

H-8020.0001 1.0mg
EUR 441
Description: Sum Formula: C83H132N26O24; CAS# [112173-48-5]

Angiogenin (108-123)

H-8020.0005 5.0mg
EUR 1675
Description: Sum Formula: C83H132N26O24; CAS# [112173-48-5]

Angiogenin (108-122)

H-8095.0001 1.0mg
EUR 212
Description: Sum Formula: C78H125N25O23; CAS# [112173-49-6]

Angiogenin (108-122)

H-8095.0005 5.0mg
EUR 756
Description: Sum Formula: C78H125N25O23; CAS# [112173-49-6]

hCRP FRET 96

CH1021-96 1 Kit
EUR 455

hCRP TRF 96

CHTR1021-96 1 Kit
EUR 482

hAlbumin FRET 96

AH1005-96 1 Kit
EUR 455

hAlbumin TRF 96

AHTR1005-96 1 Kit
EUR 482

hInsulin FRET 96

IH1017-96 1 Kit
EUR 455

hInsulin TRF 96

IHTR1017-96 1 Kit
EUR 482

rIgG FRET 96

RI1024-96 1 Kit
EUR 455

mIgG TRF 96

MIGTR1020-96 1 Kit
EUR 482

Skt ELISA Kit| Mouse Sickle tail protein ELISA Kit

EF014796 96 Tests
EUR 689

Acrp30 (108-244) Adiponectin (108-244 a.a.) Human Recombinant Protein

PROTQ15848-1 Regular: 25ug
EUR 317
Description: Acrp30 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (108-244 a.a.) and having a molecular mass of 18.1kDa.;Acrp30 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Angiogenin (108-122) (TFA)

HY-P1516A 10mM/1mL
EUR 1988

HIST1H2BC (Ab-108) Antibody

1-CSB-PA010403OA108nacHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H2BC (Ab-108). Recognizes HIST1H2BC (Ab-108) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

HIST1H2BC (Ab-108) Antibody

1-CSB-PA010403OA108nforHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H2BC (Ab-108). Recognizes HIST1H2BC (Ab-108) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IP; Recommended dilution: WB:1:200-1:2000, IHC:1:10-1:100, IP:1:200-1:2000

HIST1H2BC (Ab-108) Antibody

1-CSB-PA010403OA108nhibHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H2BC (Ab-108). Recognizes HIST1H2BC (Ab-108) from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, ChIP; Recommended dilution: WB:1:200-1:2000

Anti-IGKV1OR2-108 (3A11)

YF-MA20540 100 ug
EUR 363
Description: Mouse monoclonal to IGKV1OR2-108

Rat Coiled coil domain containing protein 108(CCDC108) ELISA kit

E02C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Coiled coil domain containing protein 108(CCDC108) ELISA kit

E03C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Coiled coil domain containing protein 108(CCDC108) ELISA kit

E08C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Coiled coil domain containing protein 108(CCDC108) ELISA kit

E06C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Coiled coil domain containing protein 108(CCDC108) ELISA kit

E04C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Coiled coil domain containing protein 108(CCDC108) ELISA kit

E07C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Coiled coil domain containing protein 108(CCDC108) ELISA kit

E01C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Coiled coil domain containing protein 108(CCDC108) ELISA kit

E09C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

HSC70 ELISA Kit

SKT-106-96 1 plate of 96 wells
EUR 717
Description: Sandwich ELISA kit used for quantitate HSC70 concentration in Cell Lysates, Tissue, Serum, Whole Blood samples from Human, Avian

HSP90 alpha ELISA Kit

SKT-107-96 1 plate of 96 wells
EUR 685
Description: Sandwich ELISA kit used for quantitate HSP90 alpha concentration in Cell Lysates, Tissue, Serum, Whole Blood samples from Human, Goat, Avian

HSP27 ELISA Kit

SKT-109-96 1 plate of 96 wells
EUR 585
Description: Sandwich ELISA kit used for quantitate HSP27 concentration in Serum, Plasma, Cell Lysates, Tissue samples from Human

HSP60 ELISA Kit

SKT-110-96 1 plate of 96 wells
EUR 585
Description: Sandwich ELISA kit used for quantitate HSP60 concentration in Cell Lysates, Tissue, Serum samples from Human

HO-1 ELISA Kit

SKT-111-96 1 plate of 96 wells
EUR 585
Description: Sandwich ELISA kit used for quantitate HO-1 concentration in Cell Lysates, Tissue, Serum, Cyst fluid samples from Human, Rat

HSP65 ELISA Kit

SKT-113-96 1 plate of 96 wells
EUR 633
Description: Sandwich ELISA kit used for quantitate M bovis/M tuberculosum HSP65 concentration in human samples in Cell Lysates, Tissue, Serum samples from M bovis, M tuberculosum

Alpha B Crystallin ELISA Kit

SKT-123-96 1 plate of 96 wells
EUR 717
Description: Sandwich ELISA kit used for quantitate alpha B crystallin concentration in Cell Lysates, Tissue, Serum samples from Human

HSP70 ELISA Kit (PLANT)

SKT-124-96 1 plate of 96 wells
EUR 717
Description: Sandwich ELISA kit used for quantitate HSP70 concentration in Plant extract, Cell Lysates, Tissue, Serum, Plasma samples from Plant, Human

Nitrotyrosine ELISA Kit

SKT-126-96 1 plate of 96 wells
EUR 460
Description: Competitive ELISA kit used for quantitate Nitrotyrosine in Plasma, Serum, Cell Lysates, Urine samples from all species

Rat GRP78 ELISA Kit

SKT-137-96 1 plate of 96 wells
EUR 632
Description: Sandwich ELISA kit used for quantitate GRP78 concentration in rat samples in Tissue samples from Rat

Dibromo-tyrosine ELISA Kit

SKT-140-96 1 plate of 96 wells
EUR 566
Description: Competitive ELISA kit used to quantify free and protein-bound 3,5-dibromotyrosine in Plasma, Urine samples from all species

Cortisol EIA Kit

SKT-201-96 1 plate of 96 wells
EUR 329
Description: Competitive EIA kit used for quantitative measuring cortisol present in Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Glutathione Detection Kit

SKT-202-96 1 plate of 96 wells
EUR 428
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species

Glutathione S-Transferase Activity Kit

SKT-203-96 1 plate of 96 wells
EUR 428
Description: Enzyme Activity Fluorometric assay used for quantitative measuring the activity of glutathione S-transferase in Urine, Serum, EDTA Plasma, Heparin Plasma, Cell Lysates samples from Human, Mollusk, Fish

Glutathione Reductase Activity Kit

SKT-204-96 1 plate of 96 wells
EUR 428
Description: Enzyme Activity Fluorometric assay used for measuring glutathione reductase activity by the amount of GSH generated from the reduction of GSSG, in Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Cell Lysates samples from Human

Corticosterone EIA Kit

SKT-205-96 1 plate of 96 wells
EUR 355
Description: Sandwich EIA kit used to measure the corticosterone present in Serum, EDTA Plasma, Heparin Plasma, Urine, Tissue Culture Media, Dried Fecal Samples samples from all species

Cortisone CLIA Kit

SKT-206-96 1 plate of 96 wells
EUR 467
Description: Sandwich CLIA kit used for quantitative measuring the cortisone present in Dried Fecal Samples, Serum, Saliva, Urine, Plasma, Tissue Culture Media samples from all species

Prostaglandin E2 EIA Kit

SKT-207-96 1 plate of 96 wells
EUR 527
Description: Sandwich EIA kit used for quantitative measuring PGE2 present in Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic AMP CLIA Kit (High-Sensitivity)

SKT-208-96 1 plate of 96 wells
EUR 494
Description: Direct CLIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic AMP EIA Kit

SKT-209-96 1 plate of 96 wells
EUR 454
Description: Direct EIA kit used for quantitative measuring cAMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Heparin Plasma, Tissue Culture Media samples from all species

Cyclic GMP CLIA Kit (High-Sensitivity)

SKT-210-96 1 plate of 96 wells
EUR 520
Description: Direct CLIA kit used to measure cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

Cyclic GMP EIA Kit

SKT-211-96 1 plate of 96 wells
EUR 474
Description: Direct EIA kit used for quantitative measuring cGMP present in Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media samples from all species

Nitric Oxide Detection Kit

SKT-212-96 2 plates of 96 wells
EUR 342
Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species

Retinol Binding Protein Urinary EIA Kit

SKT-218-96 1 plate of 96 wells
EUR 527
Description: Sandwich EIA kit used to measure RBP levels in urine samples in Urine samples from Human, Rat, Dog, Monkey

Cystatin C EIA Kit

SKT-219-96 1 plate of 96 wells
EUR 494
Description: Sandwich EIA kit used to measure cystatin C levels in Serum, EDTA Plasma, Heparin Plasma, Urine, Tissue Culture Media samples from Human

HSP70 ELISA Kit

SKT-105-96 1 plate of 96 wells
EUR 581
Description: Sandwich ELISA kit used for quantitate HSP70 concentration in Cell Lysates, Tissue, Serum, Urine samples from Human, Goat, Dog

Tissue Culture Plate, 96 Well

TCP20-96 1 UNIT
EUR 53.48

Aligned Nanofibers 96 Well Plate

3D00012-96 700 nm-PCLs
EUR 110

Bridge-It cAMP-PDE 96

PD1016-96 1 Kit
EUR 455

Guinea pig Coiled coil domain containing protein 108(CCDC108) ELISA kit

E05C1011-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Guinea pig Coiled coil domain containing protein 108(CCDC108) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Transmembrane Protein 108 (TMEM108) Antibody

20-abx116235
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Transmembrane Protein 108 (TMEM108) Antibody

abx034435-400ul 400 ul
EUR 523

Transmembrane Protein 108 (TMEM108) Antibody

abx034435-80l 80 µl
EUR 286

MHC I(108-2C5) Antibody

BNCA1095-250 250uL
EUR 383
Description: Primary antibody against MHC I(108-2C5), APC conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC811095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF680R conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC811095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF680R conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCAP1095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCAP1095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCB1095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), Biotin conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCB1095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), Biotin conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC471095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF647 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC471095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF647 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC551095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF555 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC551095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF555 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC431095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF543 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC431095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF543 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC881095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF488A conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC881095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF488A conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC941095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF594 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC941095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF594 conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCH1095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCH1095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNCP1095-250 250uL
EUR 383
Description: Primary antibody against MHC I(108-2C5), PerCP conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC041095-100 100uL
EUR 199
Description: Primary antibody against MHC I(108-2C5), CF405S conjugate, Concentration: 0.1mg/mL

MHC I(108-2C5) Antibody

BNC041095-500 500uL
EUR 544
Description: Primary antibody against MHC I(108-2C5), CF405S conjugate, Concentration: 0.1mg/mL

Saturation of tumor uptake at a high dose of Ec1 in SKOV-3 model provided a rationale for dose selection in further studies using therapeutic conjugates of Ec1 for targeted therapy. In conclusion, [125I]I-PIB-Ec1 is a promising agent for visualizing EpCAM expression in OC.

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